Deep Cholesterol Binding Sites    Anthony Lee - University of Southampton

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Deep Cholesterol Binding Sites

• Introduction
• GPCR Class A
• Rhodopsin and Opsin
• GPCR Class B
• GPCR Class C
• GPCR Class F
• Adiponectin Receptor
• Ion Channels
• Porins Etc.
• Transporters
• Other Membrane Proteins

Interfacial Cholesterol Binding Sites

• GPCR Class A [Part 1]
• GPCR Class A [Part 2]
• GPCR Class B
• GPCR Class C
• GPCR Class F
• Adiponectin Receptor
• TRPA-TRPN channels
• TRPP-TRPV channels

Deep Cholesterol Binding Sites on Mammalian Membrane Proteins

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Adiponectin Receptors

[With TM helix numbers: Units of E, kcals/mol with molar concentration units]

E	Donor		Acceptor		Local Residues (within 3 Å of a cholesterol –OH group)
AdipoR1, Human 3wxv
-6.0	Chl	OH	Thr312 [6]	OG1	Val308 [6]	Thr312 [6]
AdipoR1,open conformation,Human, 5lxg
-7.8	Chl	OH	Ser219 [3]	OG	Leu215 [3]	Ile216 [3]	Ser219 [3]
-6.3	Chl	OH	Thr312 [6]	OG1	Val308 [6]	Ile311 [6]	Thr312 [6]
AdipoR2, Human, 5lwy
-6.0	Chl	OH	Ser319 [6]	OG	Met315 [6]	Ala318 [6]	Ser319 [6]
AdipoR2 complex with fatty acid, Human, 5lx9
-6.4	Chl	OH	Ser319 [6]	OG	Met315 [6]	Ala318 [6]	Ser319 [6]
AdipoR2 complex with fatty acid, Human, 5lxa
-6.6	Chl	OH	Thr323 [6]	OG1	Ser319 [6]	Thr323 [6]
-6.4	Chl	OH	Ser319 [6]	OG	Met315 [6]	Ser319 [6]